CZU: 636.52/.58
https://doi.org/10.61562/mgfa2023.02
PERSPECTIVELE CRIOCONSERVĂRII MATERIALULUI
SEMINAL LA APLICAREA PRINCIPIILOR
VITRIFICĂRII CINETICE
Balan Ion, Roşca Nicolae, Balacci Sergiu, Buzan Vladimir, Furdui Vlada,
Cretu Roman, Bacu Gheorghe, Temciuc Vlad, Vîhrist Ecaterina, Filippov Artiom
Institutul de Fiziologie și Sanocreatologie, Universitatea de Stat din Moldova
e-mail:balanion@rambler.ru
Abstract: Currently, the most widely used application of sperm preservation is slow freezing, but during this process, sperm undergo cryodestruction, which reduces viability
and fertility properties. One of the alternatives to slow freezing for spermatozoa is kinetic vitrification, with emerging potential showing encouraging results for vitrification
of sperm used in assisted reproduction technologies of the animal kingdom. The application of kinetic vitrification principles is seen as a perspective methodology to different experimental models of semen cryopreservation. Kinetic vitrification, in addition to being free of permeable cryoprotectants, includes other advantages such as simplicity, speed, low cost, maintenance of important physiological parameters such as mitochondrial membrane potential and DNA integrity. Furthermore, vitrification without cryoprotectants may produce more biological and smaller transverse changes in reproductive cells. Also, the success of cryopreservation is measured by sperm motility after devitrification, where kinetic vitrification has not yet achieved satisfactory results in experiments performed on the semen of some animals. Thus, in order to elucidate some authentic statements regarding the success of vitrification on the quality of the devitrified semen of different animal species, more studies are needed to obtain sperm viability after devitrification.
PERSPECTIVELE CRIOCONSERVĂRII MATERIALULUI
SEMINAL LA APLICAREA PRINCIPIILOR
VITRIFICĂRII CINETICE
Balan Ion, Roşca Nicolae, Balacci Sergiu, Buzan Vladimir, Furdui Vlada,
Cretu Roman, Bacu Gheorghe, Temciuc Vlad, Vîhrist Ecaterina, Filippov Artiom
Institutul de Fiziologie și Sanocreatologie, Universitatea de Stat din Moldova
e-mail:balanion@rambler.ru
Abstract: Currently, the most widely used application of sperm preservation is slow freezing, but during this process, sperm undergo cryodestruction, which reduces viability
and fertility properties. One of the alternatives to slow freezing for spermatozoa is kinetic vitrification, with emerging potential showing encouraging results for vitrification
of sperm used in assisted reproduction technologies of the animal kingdom. The application of kinetic vitrification principles is seen as a perspective methodology to different experimental models of semen cryopreservation. Kinetic vitrification, in addition to being free of permeable cryoprotectants, includes other advantages such as simplicity, speed, low cost, maintenance of important physiological parameters such as mitochondrial membrane potential and DNA integrity. Furthermore, vitrification without cryoprotectants may produce more biological and smaller transverse changes in reproductive cells. Also, the success of cryopreservation is measured by sperm motility after devitrification, where kinetic vitrification has not yet achieved satisfactory results in experiments performed on the semen of some animals. Thus, in order to elucidate some authentic statements regarding the success of vitrification on the quality of the devitrified semen of different animal species, more studies are needed to obtain sperm viability after devitrification.
Keywords: animal, sperm material, vitrification, cryopreservation, cryoprotector,
reproduction.
reproduction.
